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File: Hplc Slideshare 68366 | Determination Of Metformin In Urine
determination of metformin in biological fluids human plasma urine etc challenge to conventional chromatographic methods metformin oral antidiabetic drug small molecule with high polarity impossible to maintain by rp columns ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 3 years ago

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    Determination of metformin in biological fluids
        (human plasma, urine, etc)
  Challenge to conventional chromatographic methods 
         Metformin
         •   Oral antidiabetic drug
         •   Small molecule with high polarity
         (impossible to maintain by RP columns)
         •   pKa1=2.8 and pKa2=11.5
                               Determination of Metformin in urine
                 There are many methods to determine Metformin in urine :
                 •  Capillary electrophoresis (with contactless conductivity 
                    dedection)
                 •  Voltammetric method
                 •  Cation-exchange HPLC 
                 •  Normal phase chromatography
                 •  LC-MS
                 •  RP-HPLC (specific column or common columns using ion-
                    pairing agent)
          The purpose of the study
     To developed a method using a conventional column
        Ion-pairing chromatography
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...Determination of metformin in biological fluids human plasma urine etc challenge to conventional chromatographic methods oral antidiabetic drug small molecule with high polarity impossible maintain by rp columns pka and there are many determine capillary electrophoresis contactless conductivity dedection voltammetric method cation exchange hplc normal phase chromatography lc ms specific column or common using ion pairing agent the purpose study developed a...

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Category: Uncategorized / Uncategorized
Posted on: 28 Aug 2022 | 3 years ago
Tag: download pptx, determination, metformin, urine,
Filetype: PPTX
File size: 1.44 MB
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