Figure 1. Example of original western blot for three repeats
                        Repeat 1          Repeat 2           Repeat 3
                  170                170                                      If the whole membrane was cut to blot 
      TYR 80 kDa 130                 130               170
                  100                100               130                    for different antibodies, you are required 
                   70                70                100                    to load all pieces of whole blot and the 
                                                        70
      DCT 59 kDa 70                  70                 70                    molecular marker must be shown in each 
                   55                55                 55                    blot.  Figure 1 shows the whole blot after 
     Actin 43 KDa 55                 55                 55
                   40                40                 40                    cutting membrane at molecular weight 
                                     35                 35                    70 kDa, 55 kDa, 35 kDa and 15~25 kDafor 
                   35                35                 35
    GSTP 26 KDa    25                25                 25                    TYR (80 kDa), DCT (59 kDa), actin (43 
                   15                15                                       kDa), GSTP (26 kDa) and MGST1 (17 kDa).
  MGST1 17 KDa                                          15
                   10                10
                       MNNMNM NM           NMNMNM NM         NM NMNMNM
                       T1-1-T1-T1-T        1-T1-T1-T1-T       1-T 1-T 1-T 1-T
                        TNDK TN DK         TN DK TN DK       -TN-DK-TN-DK
                       -11-2- 2-           1-1-2- 2-         1  1 2 2