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Functional Area of Physiology and Behavior Histology The Histology platform is equipped with instruments utilized for the preparation of histological samples. The instruments available allow the inclusion of biological samples in paraffin, resin or optimal cutting temperature compound (OCT) and the cutting of sections of various thickness through the use of vibratome,cryostat andmicrotome. First Floor - EAST Wing, #149, #150 Manager: Pamela Imperadore pamela.imperadore@szn.it Bookable Description instruments This cryostat is used to cut histological slides Cryostat (frozen section histology). It is a rotary LEICA CM3050 S microtome placed in a freezer. The temperature can be varied, depending on the tissue being cut - usually from - 20 to -30° C. Paraffin Rotary The instrument is a semi-motorized rotary Microtome microtome utilized for paraffin sections. LEICA RM2245 This histology oven, equipped with a Paraffin Oven VACUUBRAND vacuum pump, is useful for BINDER VD115 dissolving paraffin and keeping it in a liquid state; also used for the inclusion of samples. Dissection table equipped with two Dissectiong and stereomicroscopes, one of which is didactic, at Sorting Table lowmagnification. Vibrating blade microtome for high quality Vibratome sectioning, can be used in semi- or fully LEICA VT1200S automatedmode. The instrument produces high quality sections Sliding Microtome for routine histopathology (paraffin), LEICA SM2010R neuroscience (fixed frozen brain) and industrial applications (foam and wood). Microarray Scanner Optimal high resolution scanner for very high MOLECULAR DEVICE density microarray. This scanner acquires data at GenePix 4100A user-selectable resolutions between 2.5 and 100 μmperpixel. Benchtop Laminar It is a partially recirculating downflow cabinet. Flow Cabinet ThelaminarflowisequippedwithaUVlight. BIOAIR Book on www.labagenda.com Functional Area of Physiology and Behavior Histology The Histology platform is equipped with instruments utilized for the preparation of histological samples. The instruments available allow the inclusion of biological samples in paraffin, resin or optimal cutting temperature compound (OCT) and the cutting of sections of various thickness through the use of vibratome,cryostat andmicrotome. First Floor - EAST Wing, #149, #150 Manager: Pamela Imperadore pamela.imperadore@szn.it Bookable Description instruments Chemical Fume Chemical hood intended for the preparation and Hood - Histology processing of histological samples (e.g., fixation of sample preparation samplesforhistology,staining) Refrigerated The Innova 4230 Refrigerated/Heated Incubator Incubator Shaker Shakerhasthecapabilityto heatorrefrigerate. NEW BRUNSWICK Temperatures in this refrigerated/heated incubator SC.Innova4230 shaker can be set from a minimum of 4°C to maximumof75°C. Microplate The instrument measure absorbance in 96-well Photometer plates. It is recommended for Immunoassays ThermoFisher (ELISA), protein assays, endotoxins, cytotoxicity Scientific and proliferation assays, enzyme assays, growth curves. Multiskan™ FC The Leica Z16 APO is a fully apochromatic zoom Macroscope system for high contrast, high-resolution, detailed Leica Z16 APO analysis. The single beam path provides 2D images andensuresparallax-freeimaging. Refrigerated The Eppendorf 5810 R refrigerated centrifuge is Centrifuge equipped with tilting rotors and adapters that Eppendorf5810 R allow to accommodate test tubes of various sizes, from02mLto50mL. Refrigerated The Allegra 21R is a compact benchtop centrifuge Centrifuge reaching speeds up to 15,300 rpm and has a Beckman Coulter temperaturerangeof-20°to40°C. Allegra 21R Oven with a temperature range between 50 and 350°C. Laboratory Oven Book on www.labagenda.com
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