Joint Committee for Traceability in Laboratory 
       Medicine (JCTLM) Meeting
                      Blood Cell Counting 
                      Blood Cell Counting 
                          Standardization
                          Standardization
                                 November 15, 2005
                                 November 15, 2005
                               Keiji FUJIMOTO
                     Vice President, Dept. of Scientific Affairs 
                     Vice President, Dept. of Scientific Affairs 
                               Sysmex Corporation 
                               Sysmex Corporation 
                   Member of ICSH Expert Panel on Cytometry
                   Member of ICSH Expert Panel on Cytometry
                                    Copyright(c) 2005 SYSMEX CORPORATION, All Rights Reserved. 
                    Contents
                    Contents
      ¾ Traceability of Blood Cell Counting
      ¾ Traceability of Blood Cell Counting
        ¾ICSH Reference Methods 
        ¾ICSH Reference Methods 
      ¾ Reference Method for Red Blood Cell Counting 
      ¾ Reference Method for Red Blood Cell Counting 
        ¾Measurement Principle
        ¾Measurement Principle
        ¾Traceability Chain 
        ¾Traceability Chain 
        ¾Estimation of Uncertainty in Measurement
        ¾Estimation of Uncertainty in Measurement
      ¾ Present Situation of Standardization
      ¾ Present Situation of Standardization
        ¾6 manufacturers’ comparison study in Japan
        ¾6 manufacturers’ comparison study in Japan
      ¾ Summary
      ¾ Summary
           List of Reference Methods(1/4)
         RBC & WBC
         Reference method for the enumeration of erythrocytes
         and leucocytes
         International Council for Standardization in Haematology;  Prepared by the 
        Expert Panel on Cytometry
         - Clinical Laboratory Haematology, 16, 131-138 (1994)
                                                             3
            List of Reference Methods(2/4)
          Platelet
          Platelet Counting by the RBC/Platelet Ratio Method
          A Reference Method       （CD41/61)
          International Council for Standardization in Haematology Expert Panel on   
         Cytometry and International Society of Laboratory Hematology Task Force on 
         Platelet Counting 
          - American Journal  of Clinical Pathology 115:460-464 (2001)
                                             PLT
                                                     RBC
                            RBC/PLT ratio
                                                                     4