Nucleic acid
      extraction and 
      purification 
      technologies
      For research use only.  
      Not for use in diagnostic procedures
                 Extraction and                                                                                                                                                                                                 DNA extraction 
                 purification solutions                                                                                                                                                                                         chemistries
                 from LGC, Biosearch                                                                                                                                                                                            QuickExtract
                 Technologies                                                                                                                                                                                                   For DNA screening and genotyping-type 
                                                                                                                                                                                                                                applications, PCR-grade genomic DNA is 
                                                                                                                                                                                                                                sufficient, which can be rapidly and efficiently 
                 Biosearch Technologies is unique in the nucleic                                                            DNA extraction chemistries                                                                          extracted using QuickExtract™ DNA Extraction 
                 acid extraction and purification marketplace,                                                   3 QuickExtract                                                                                                 Solution. In 3-8 minutes, and using only a single-
                 in that we are both a service provider, and                                                                                                                                                                    tube protocol, PCR-grade genomic DNA from 
                 a developer of proprietary chemistries and                                                                 DNA purification chemistries                                                                        almost any sample type can be extracted, without 
                 instrumentation. We develop chemistries and                                              4-6 PCR-grade purifications – Kleargene                                                                               the use of centrifuges or spin-columns (Figure 1).
                 instrumentation for customers to use in their                                                              NGS-grade purifications – sbeadex (plant,                                                                                                        Add samples
                 own laboratories, and it is these same products                                                            livestock, blood)                                                                                                                                                                           QuickExtract Solution has been used to 
                 which we use in each of our service laboratories.                                                                                                                                                                                                                                                      extract DNA from samples such as hair 
                 This allows Biosearch Technologies to be highly                                                 7 DNA/RNA/total nucleic acid                                                                                                                                                                           follicles, quill-end cells of feathers, tissue-
                 flexible, and offer individual solutions for each of                                                       purification chemistries                                                                                                                                      QuickExtract                  culture cells, buccal cells, zebrafish organs 
                 our customers.                                                                                             MasterPure                                                                                                                                                    solution                      and scales, and mouse tail snips. The 
                 To provide the most efficient and effective                                                                                                                                                                                                        Heat at 65 ˚C for 6 minutes                         extracted DNA is suitable for PCR analyses 
                 extraction or purification method, we offer not                                                 8 Automation                                                                                                                                         and 98 ˚C for 2 minutes                           (Figure 2), such as genomic, transgenic, 
                 only a range of different technologies but also the                                                                                                                                                                                                                                                    or viral DNA screening in animals, or for 
                 capability to develop tailor-made kit systems for all                                                      Purification services                                                                                                                                                                       genetic or environmental research and 
                 applications and downstream requirements.                                                       9 DNA purification services                                                                                                                                              PCR-ready                     screening in humans and other organisms.
                                                                                                                                                                                                                                 Figure 1: Procedure for obtaining                        DNA
                                                                                                                            RNA purification services                                                                            PCR-ready DNA using QuickExtract 
                 Our range of technologies allows delivery of                                                                                                                                                                    DNA Extraction Solution.
                 optimised extractions and purifications from an                                            10 Overview 
                 unrivalled array of matrices including:                                                                                                                                                                         The range of QuickExtract Extraction Solutions for 
                 •  Plant material (including leaves, seeds, and                                             11 Order information                                                                                                additional sample types includes:
                     other plant matrices)                                                                                                                                                                                       •  QuickExtract DNA Extraction Solution
                 •  Livestock samples (including blood, animal                                                                                                                                                                   •  QuickExtract FFPE DNA Extraction Solution
                     tissue, hair follicle, buccal swabs and saliva)                                                                                                                                                             •  QuickExtract Plant DNA Extraction Solution
                 •  Human samples (including blood, tissue and                                                                                                                                                                   •  QuickExtract RNA Extraction Solution
                     forensic samples)
                 •  Plasmid preparations
                 •  Bacteria, prokaryotes and other                                                                                                                                                                              Figure 2: Samples extracted with QuickExtract DNA Extraction Solution. 
                     microorganisms                                                                                                                                                                                              PCR was performed using primers to amplify the regions indicated: Lanes 
                                                                                                                                                                                                                                 1-3, human β-globin; lane 4, transgenic mouse GAPDH; lane 5, E. coli 16S 
                                                                                                                                                                                                                                 ribosomal RNA gene; lane 6, transgenic SV40 T antigen.
         2                                                                                                                                                                                                                                                                                                                                                                                                            3
                                                                                                                                                                                     need of a drying stage, and minimising any                              sbeadex plant
             DNA purification                                                                                                                                                        contaminating ethanol carryover into the final 
                                                                                                                                                                                     eluate. The switch between binding mechanisms                           Plant samples are often challenging due to 
                                                                                                                                                                                     also allows for greater purity of nucleic acids.                        secondary metabolites, high levels of sugars and 
             chemistries                                                                                                                                                             The advantages of sbeadex include:                                      polyphenols, or robust cell walls. sbeadex plant 
                                                                                                                                                                                                                                                             protocols for leaf and/or seed material from a broad 
                                                                                         Kleargene has been highly optimised for                                                     •  High-quality DNA: suitable for all genomic-                          range of plant species have been validated and 
              PCR-grade DNA                                                              automation with both 96-well formats (plant and                                                applications (including NGS)                                         can be automated on all open platforms, including 
                                                                                         tissue) and 384-well formats (plant), achieving                                             •  Compatible with Biosearch Technologies                               the Biosearch Technologies oKtopure. Validated 
              purifications                                                              higher throughput than comparable purification                                                 automated Oktopure platform.                                         sbeadex purification protocols have been developed 
                                                                                         technologies.                                                                               •  Automation-friendly: suitable for most popular                       for the following plant species (Table 3):
              Kleargene                                                                  The advantages of Kleargene include:                                                           magnetic-based robotic platforms                                         Plant species with validated sbeadex 
                                                                                                                                                                                     •  Tailor-made lysis conditions according to                                purification protocols
                                                                                                                                                                                        customer requirements
              Kleargene purification chemistry uses spin-                                •  High throughput DNA purifications (96-well                                               •  Quicker results: purification-time optimisation                          Aphid, soybean       Cucumber                Rice, Asian (Oryza 
              plates and a glass-fibre support to purify DNA                                and 384-well formats)                                                                    •  Flexible batch sizes and kit volumes                                     (Aphis glycines)     (Cucumis sativus)       sativa)
              using polar interactions to generate PCR-grade                             •  Semi-automated (via Biosearch Technologies                                               •  No organic solvents in final wash buffer: results                        Aubergine            Pepper (Capsicum        Sunflower 
                                                                                                                                                                                                                                                                 (Solanum             annuum)                 (Helianthus 
              DNA, applicable with high throughput methods.                                 Genespin platform)                                                                          in higher purity                                                         melongena)                                   annuus)
              Protocols have been optimised for both plant and                           •  Manual high throughput purifications (via                                                •  No salts in eluates: high                                                Barley                                       Tomato 
                                                                                                                                                                                                                       OD260/230                                 (Hordeum             Potato (Solanum         (Solanum 
              animal purifications, and the Kleargene chemistry                             centrifugation)                                                                                                                                                      vulgare)             tuberosum)              lycopersicum)
              can generate yields between 100 ng and 10 µg                               •  Broad range of sample types (plant leaves,                                                 sbeadex feature           Specification                                                        Rapeseed/Canola/
              DNA from samples ranging from plant leaf tissue                               plant seeds, animal tissue)                                                                Colour                    Dark brown                                      Beet, sugar          Oilseed (Brassica       Wheat (Triticale)
              and plant seeds to rodent tails (Figure 4).                                •  High yield of DNA (PCR-grade)                                                                                                                                        (Beta vulgaris)      napus)
                                                                                                                                                                                       Superparamagnetic         Proprietary double-coated 
                                                                                                                                                                                       bead structure            technology (competitor: single-             Table 3: Examples of plant species, which have validated purification 
                                                                                                          Amount of                                                                                              coated)                                     protocols using sbeadex chemistry.
                                      Kit                   Application areas                             starting material          Expected yield                                                              Irregular
                                                                                                                                                                                       Size and shape            80% 5-10 µm (diameter); a; <53 µm                                               Amount 
                                      Kleargene spin        Faster discovery of new traits resulting      >6 leaf punches            5 µg                                                                                                                       Kit          Application         of starting      Expected 
              Table 1: Scales         96-well plates        in faster to market. No laboratory at your                                                                                 Maximum capacity          2 µg DNA/mg sbeadex particle                                areas               material         yield
              of available            Kleargene spin        location required                             2-3 leaf punches           1.5 µg                                            Binding material and      2-step binding mechanism: first
              Kleargene kits.         384-well plates                                                                                                                                  technology                silica and second DNA adaptor                   sbeadex 
                                                                                                                                                                                                                                                                 mini                             20 – 30 mg        2 µg
                                                                                                                                                                                       Purification format       96                                              plant         Plant leaves; 
                                                                                         generation sequencing applications can be met                                                                                                                           sbeadex       Plant seeds
              Next-generation                                                                                                                          ®                               Automation                KingFisher (lab validated); applicable          maxi                             80 – 100 mg       10 µg
                                                                                         by Biosearch Technologies portfolio of sbeadex                                                                          with alternative open liquid handlers           plant
              sequencing-grade                                                           chemistries. All sbeadex kits use surface modified                                                                      Blood; plant; livestock; forensics; 
                                                                                         superparamagnetic particles that bind nucleic                                                 Sample material           plasmids; other human samples               Table 4: Scales of available sbeadex plant kits.
                                                                                         acids via a novel, two-step binding mechanism                                                                                       ®      ®
              purifications                                                                                                                                                                                      PCR; KASP ; BHQ  Probe 
                                                                                         (Figure 3). Firstly, the nucleic acids affix to the                                           Application               chemistry; Sanger; NGS; 
                                                                                         beads via polar interactions, and secondly, the                                                                         Microarrays
              sbeadex                                                                    nucleic acids are bound via an affinity-driven                                                Storability               >12 months
                                                                                         mediator. This second binding step allows the final                                         Table 2: Features and specification of sbeadex beads.
              The requirement to generate high quality and                               wash steps to be carried out using water-based 
              quantity DNA for PCR, sequencing and next-                                 wash solutions, eliminating the                                                             sbeadex chemistry can be used for a variety of 
                                                                                                                                                                                     species, by modifying the lysis conditions. Although 
              Figure 3: Standard work                                                                                                                                                our standard protocols already deliver high quality 
              flow for all sbeadex-based                                                                                                                                             DNA, sbeadex can be customised and optimised 
              purification chemistry                                                                                                                                                 according to customer requirements.
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               sbeadex livestock                                                                                                                                                                           DNA/RNA/total  
               The majority of NGS-based genomic analysis 
               technologies (including those working with 
               livestock samples) typically require high-quality                                                                                                                                           nucleic acid purification  
               DNA preparations which are often challenging 
               and labour-intensive to produce. sbeadex 
               livestock is based on one protocol which can                                                                                                                                                chemistries
               be used for all sample types and the oKtopure 
               can be used for all livestock samples (excluding 
               blood).                                                                                                                                                                                     MasterPure                                                                           •  Genomic DNA and cDNA cloning
                                                                                                                                                                                                                                                                                                •  PCR, qPCR, RT-PCR and qRT-PCR
                                                                                                                                                                                                                                                                                                •  Microarray analysis (including CGH and gene 
                                                                                                    sbeadex blood                                                                                          Some applications require flexibility of isolating                                       expression profiling)
                       Sample type                        Animal species                                                                                                                                   high-molecular weight genomic DNA, total 
                                                                 Bovine                             Blood samples can often vary considerably in                                                           cellular RNA or total nucleic acid from the same                                     The kit uses a scalable salt-precipitation protocol 
                            Blood*                              Chicken                                                                                                                                    sample. The MasterPure™ Complete DNA and                                             which eliminates the need for hazardous 
                         Hair follicle                            Dog                               quality, and this can lead to wide variations in the                                                   RNA Purification Kit allows for isolation of NGS-                                    chemicals (no need for phenol, chloroform or 
                            Saliva                                Fish                              consistency and yield of extracted DNA. sbeadex 
                            Semen                                 Goat                              blood provides high yields of high-molecular                                                           grade DNA, RNA or total nucleic acid in 30–60                                        guanidine). Depending on the amount of nucleic 
                   Tissue (e.g. ear punch)                       Horse                                                                                                                                     minutes using a single kit, and is suitable for many                                 acid required, and size of the sample, different 
                         Buccal swab                             Sheep                              weight, double-stranded DNA from different                                                             different sample types.                                                              MasterPure kits are available, including:
                          FTA cards                                Pig                              blood preparations including those treated with 
                                                                                                    anticoagulants, including:                                                                             Applications for the MasterPure kit include:                                         •  MasterPure Complete DNA and RNA 
               Table 5: Sample types from different species applicable with the sbeadex                                                                                                                    •  Library preparation for next-generation                                               Purification Kit
               livestock kit. *Not compatible with the Oktopure.                                    •  EDTA blood
                                                                                                    •  Heparin blood                                                                                           sequencing of genomic DNA and RNA                                                •  MasterPure DNA Purification Kit for Blood
                                                                                                    •  Citrate blood                                                                                       •  DNA methylation studies (for example, with use                                    •  MasterPure Gram Positive DNA Purification Kit
                                                                                                                                                                                                                                      ®             ®
                                                                                                    •  Buffy coat preparations                                                                                 with the Illumina  Infinium  HumanMethylation                                    •  MasterPure Yeast RNA Purification Kit
                                                                                                                                                                                                               BeadChips)
                                                                                                                    Application  Amount                       Expected 
                                                                                                       Kit          areas                of starting          yield                                        Large volume DNA purifications
                                                                                                                                         material
                                                                                                     sbeadex         Whole blood;                                                                          Biobanking and other human research markets                                      offers two cost-effective, easy and rapid methods for 
                                                                                                     blood           buffy coat           100-200 µL        4-12 µg                                        require large volume purification technologies which                             the purification of DNA from large volume samples 
                                                                                                                     preparations
                                                                                                                                                                                                           provide high quality DNA. Biosearch Technologies                                 (Table 9):
                                                                                                    Table 6: Scales of available sbeadex blood kits.
                                                                                                                                                                                                                   Features                             Kleargene XL                                         PLUS XL
                                                                                                                                                                                                                                           Detergent-driven cell lysis, followed by            DNA cell-components are enriched, and 
                                                                                                                                                                                                                                           guanidine-based purification with DNA               residual proteins are digested away. 
                                                                                                                                                                                                               Technology                  binding to silica particles. Contaminants are       The DNA is subsequently precipitated, 
                                                                                                                                                                                                                                           removed using organic solvent-based wash            washed, dried, and then resuspended.
                                                                                                                                                                                                                                           steps, followed by elution.
                                                                                                                                                                                                               Automation                  Manual purifications only                           Manual purifications only
                                                                                                                                                                                                               Applications                NGS-grade                                           NGS-grade
                                                                                                                                                                                                               Starting volume             Scalable: Up to 10 mL                               Scalable: Up to 10 mL
                                                                                                                                                                                                               Sample types                Whole blood; Saliva (including Oragene              Whole blood; Saliva (including Oragene 
                                                                                                                                                                                                                                           collection tubes); Tissue;                          collection tubes); Tissue;                     Table 7: Key features 
                                                                                                                                                                                                               Typical DNA yields          10-800 µg                                           10-800 µg                                      of large volume DNA 
                                                                                                                                                                                                                                                                                                                                              purification chemistries.
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