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picture1_Leishman Staining Procedure Pdf 87429 | Staining Procedure Thin Thick Film


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File: Leishman Staining Procedure Pdf 87429 | Staining Procedure Thin Thick Film
staining procedure for thin blood film 1 when the blood smear is totally dry arrange the slides on the slide rack with the blood smear facing front the slides must ...

icon picture PDF Filetype PDF | Posted on 14 Sep 2022 | 3 years ago
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               Staining procedure for thin blood film 
                    1.  When the blood smear is totally dry, arrange the slides on the slide rack,     with 
                       the blood smear facing front. The slides must not be in contact with   each other or 
                       else the stain will be flow off. 
                    2.  Cover each slide completely with 1-2ml of Leishman’s stain (undiluted)         using 
                       Pasteur pipette and leave for around 45seconds. 
                    3.  Add the buffer. Mix the stain by blowing gently to and fro. 
                    4.  Leave the slides to stain for 17 minutes. (The time can be modified if  necessary, 
                       with different batches of stains – from 12 to 20 minutes) 
                    5.  At the end of 17 minutes, wash the stain off by pouring tap water onto the   slides 
                       from one side and rub off the remaining stain at the back of the slides by using a 
                       sponge. 
                    6.  Put the slides at the slide stand for air dry. 
               Staining procedure for thick blood film 
                       1.  Place the thick smear slide flat on a staining rack. Carefully overlay the entire 
                          smear with demineralized water. 
                       2.  Allow the water to evaporate and the slide to air dry completely. DO not heat or 
                          disturb the slide during this phase. 
                       3.  Dip the dried smear in Giemsa Plus fixative 5 times, 1 second per dip and drain 
                          the excess. 
                       4.  Then, dip the slide in Giemsa Plus Reagent A 5 times, 1 second per dip. Drain the 
                          excess. 
                       5.  Lastly, dip the slide in Giemsa Plus Reagent B 5 times, 1 second per dip. 
                       6.  Rinse the smear with distilled water and allow to air dry. 
                        
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...Staining procedure for thin blood film when the smear is totally dry arrange slides on slide rack with facing front must not be in contact each other or else stain will flow off cover completely ml of leishman s undiluted using pasteur pipette and leave around seconds add buffer mix by blowing gently to fro minutes time can modified if necessary different batches stains from at end wash pouring tap water onto one side rub remaining back a sponge put stand air thick place flat carefully overlay entire demineralized allow evaporate do heat disturb during this phase dip dried giemsa plus fixative times second per drain excess then reagent lastly b rinse distilled...

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