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picture1_Specimen Collection Ppt 83393 | Pracangstaph


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File: Specimen Collection Ppt 83393 | Pracangstaph
collection of specimens the type of specimen required for the diagnosis of s aureus depends on the site of the infection patients contribute a urine sample for the diagnosis of ...

icon picture PPT Filetype Power Point PPT | Posted on 12 Sep 2022 | 3 years ago
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              Collection of Specimens
  •  The type of specimen required for the diagnosis of S. aureus 
     depends on the site of the infection. 
  •  Patients contribute a urine sample for the diagnosis of urinary tract 
     infections. 
  •  For blood infections, lab technicians withdraw blood from the patient 
     using a syringe and transfer it to a blood culture bottle.
   
  •  Swabs, which consist of a sterile absorbent material such a cotton, 
     are used to collect specimens from throat, nostrils, skin, wounds and 
     other accessible lesions. 
                                          TASKS 
  group 1                  group  2                      group  3                       group  4
  S. aureus – GRAM         S. epidermidis – GRAM         S. aureus +                    S. aureus - GRAM
                                                         S. epidermidis -GRAM
  G+ and G- cell wall  Describe the GRAM                                                ATB susceptibility 
  structure                staining procedure            ATB susceptibility test:       test: E-test - 
                                                         Disc diffusion Test -          principle
                                                         principle
  S. aureus –              S. epidermidis -              S. aureus –                    S. epidermidis - 
  cultivation on           cultivation on blood          catalase test                  catalase test 
  blood agar-              agar – describe               Describe principle of 
  describe colonies        colonies                      catalase test 
  S. aureus –              S. epidermidis -              S. aureus – bound              S. epidermidis – 
  cultivation on salt      cultivation on salt           plasmacoagulase test           Describe principle 
  mannitol medium          mannitol medium –             (clumping factor)              of free 
                           describe the principle                                       plasmacoagulase 
                                                                                        test
        Microscopy
  • Cuture of Staphylococcus aureus - fixed 
   and stained by Gram:                                  
                         - G+ cocci in clusters
       sputum - staphylococci + 
              pneumococci
                       staphylococci
      leucocyt
                           pneumococci
  Pus – intracellular localization 
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...Collection of specimens the type specimen required for diagnosis s aureus depends on site infection patients contribute a urine sample urinary tract infections blood lab technicians withdraw from patient using syringe and transfer it to culture bottle swabs which consist sterile absorbent material such cotton are used collect throat nostrils skin wounds other accessible lesions tasks group gram epidermidis g cell wall describe atb susceptibility structure staining procedure test e disc diffusion principle cultivation catalase agar colonies bound salt plasmacoagulase mannitol medium clumping factor free microscopy cuture staphylococcus fixed stained by cocci in clusters sputum staphylococci pneumococci leucocyt pus intracellular localization...

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