251x Filetype PPT File size 2.88 MB Source: www.jfmed.uniba.sk
Collection of Specimens • The type of specimen required for the diagnosis of S. aureus depends on the site of the infection. • Patients contribute a urine sample for the diagnosis of urinary tract infections. • For blood infections, lab technicians withdraw blood from the patient using a syringe and transfer it to a blood culture bottle. • Swabs, which consist of a sterile absorbent material such a cotton, are used to collect specimens from throat, nostrils, skin, wounds and other accessible lesions. TASKS group 1 group 2 group 3 group 4 S. aureus – GRAM S. epidermidis – GRAM S. aureus + S. aureus - GRAM S. epidermidis -GRAM G+ and G- cell wall Describe the GRAM ATB susceptibility structure staining procedure ATB susceptibility test: test: E-test - Disc diffusion Test - principle principle S. aureus – S. epidermidis - S. aureus – S. epidermidis - cultivation on cultivation on blood catalase test catalase test blood agar- agar – describe Describe principle of describe colonies colonies catalase test S. aureus – S. epidermidis - S. aureus – bound S. epidermidis – cultivation on salt cultivation on salt plasmacoagulase test Describe principle mannitol medium mannitol medium – (clumping factor) of free describe the principle plasmacoagulase test Microscopy • Cuture of Staphylococcus aureus - fixed and stained by Gram: - G+ cocci in clusters sputum - staphylococci + pneumococci staphylococci leucocyt pneumococci Pus – intracellular localization
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