The following hybridization mix is prepared for each sample
      Fragmented cRNA              5ug                                          10 ul       
       Control B2 Oligo                                                         1.7 ul
      20x Eukaryotic Control mix [bio B, bio C, bio D, Cre]                      5 ul 
      Herring Sperm DNA [10mg/ml]                                                1 ul 
      Acetyleted BSA [50mg/ml]                                                   1 ul
      DMSO                                                                      10 ul
      2x Hybridization Buffer                                                   50 ul
      Water                                                                     22.3 ul
                                          Denature 99C               Inject into
                                          10 minutes                 GeneChip
                                                       
             The hybridization oven
                Target binds to the Probes
                              
              RNA-DNA Hybridization
    Targets:
    Antisense 
    biotinylated
     cRNA
       Probe sets: The DNA oligo probe is attached to the 
                    GeneChip via a silane bond
                                  
                                  Hybridization
         Optimized Hybridization is the process of single stranded nucleic acids binding 
               to another strand with identically complement sequence [We hope] 
                                      Types:   DNA to DNA     
                                                DNA to RNA 
                                                RNA to RNA 
                                                PNA to DNA 
                                                              
                                              
                                            
                    Stringency  
     Stringency is a condition that causes a change in the local 
    hybridization environment and “interferes” with the binding kinetics
    Stringency prevents: 
    .    Binding of non-complementary strands 
         Self hybridization – hairpin formation
         Disassociation of strands