Introduction
       This presentation introduces gel permeation chromatography (GPC)
       The mechanism of GPC shall be discussed
       The method of analysing data shall be introduced
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                                   Types of Liquid Chromatography
                    Interactive adsorption, partition, ion exchange, etc
                             Non-interactive GPC, SEC, GFC
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                                                      Nomenclature
                   Gel Permeation Chromatography GPC
                   Size Exclusion Chromatography SEC
                   Gel Filtration Chromatography GFC
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                                                        What is a GPC System?
                                     1.   Reciprocating piston pump delivers eluent from 
                                          reservoir  at  a  constant  volumetric  flow  rate. 
                                          GPC is always an isocratic separation
                                     2.   Two     position    injection    valve    permits 
                                          introduction   of    sample    solution    without 
                                          interrupting  solvent  flow.  GPC  tends  to  use 
                                          larger injection volumes than HPLC (typically up 
                                          to 200ul)
                                     3.   GPC columns perform a separation based on 
                                          the  molecular  size  of  polymer  molecules  in 
                                          solution.  Resolution  and/or  resolving  range  is 
                                          increased by use of multiple column systems
                                     4.   Detector responds to concentration of polymer 
                                          molecules eluting from the GPC columns
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                                        Additional Components Used in GPC
         Concentration detectors
         
             Differential refractometer (RI)
         
             Ultraviolet absorbance (UV)
         
             Evaporative light scattering or mass detector (ELS, EMD)
         
             Infra-red (IR)
         Molecular weight sensitive detectors
         
             Viscometry 
         
             Light scattering
         Additional systems
         
            Online degasser
         
            Autosampler
         
            Column oven
         
            Additional specific detectors
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