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picture1_Ppt Physical Therapy 77102 | 421 821 Chapter 26


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File: Ppt Physical Therapy 77102 | 421 821 Chapter 26
the analytes interacting most strongly with the stationary phase will take longer to pass through the system than those with weaker interactions these interactions are usually chemical in nature but ...

icon picture PPT Filetype Power Point PPT | Posted on 02 Sep 2022 | 3 years ago
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                                                                     The  analytes  interacting  most 
                                                                     strongly   with   the   stationary 
                                                                     phase  will  take  longer  to  pass 
                                                                     through  the  system  than  those 
                                                                     with weaker interactions.
                                                                     These  interactions  are  usually 
                                                                     chemical in nature, but in some 
                                                                     cases physical interactions  can 
                                                                     also be used.
    Types of Chromatography
    1.) The primary division of chromatographic techniques is based on the type of mobile phase 
        used in the system:
             Type of Chromatography              Type of Mobile Phase
             Gas chromatography (GC)                      gas
             Liquid chromatograph (LC)                    liquid
    2.) Further divisions can be made based on the type of stationary phase used in the system:
                               Gas Chromatography
                               Gas Chromatography
             Name of GC Method                   Type of Stationary Phase
             Gas-solid chromatography            solid, underivatized support
             Gas-liquid chromatography           liquid-coated support
             Bonded-phase gas chromatography     chemically-derivatized support
    Types of Chromatography
                      Liquid Chromatography
                      Liquid Chromatography
        Name of LC Method                         Type of Stationary Phase
        Adsorption chromatography                 solid, underivatized support
        Partition chromatography                  liquid-coated or derivatized support
        Ion-exchange chromatography               support containing fixed charges
        Size exclusion chromatography             porous support
        Affinity chromatography                   support with immobilized ligand
  3.) Chromatographic techniques may also be classified based on the type of support material 
    used in the system:
                 Packed bed (column) chromatography
                Open tubular (capillary) chromatography
                 Open bed (planar) chromatography
    Theory of Chromatography
    1.) Typical response obtained by chromatography (i.e., a chromatogram):
               chromatogram  - concentration versus elution time
                                                                          W
                                                                            h
                                                                          W
                                                                            b
             Inject
                    Where:
                              tR = retention time
                              t  = void time
                               M
                              W = baseline width of the peak in time units
                                 b
                              W = half-height width of the peak in time units
                                 h
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...The analytes interacting most strongly with stationary phase will take longer to pass through system than those weaker interactions these are usually chemical in nature but some cases physical can also be used types of chromatography primary division chromatographic techniques is based on type mobile gas gc liquid chromatograph lc further divisions made name method solid underivatized support coated bonded chemically derivatized adsorption partition or ion exchange containing fixed charges size exclusion porous affinity immobilized ligand may classified material packed bed column open tubular capillary planar theory typical response obtained by i e a chromatogram concentration versus elution time w h b inject where tr retention t void m baseline width peak units half height...

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