154x Filetype PPTX File size 0.26 MB Source: abu.edu.iq
Uses Identification Further studies (e.g., Pathogenicity, antiviral sensitivity, research) Limitations Absence of detection system for the agent Inappropriate culture systems Viruses that cannot be cultured A negative viral culture results does not mean that the agent is absent Specimens used to culture viruses Blood specimens Stools, rectal swabs EDTA Urine Heparin Saliva Serum Cerebro-spinal fluid Stool Biopsy Throat swabs Skin (filoviridae) Naso-paryngeal aspirates Organs (fixation with formaldehyde 10%) Virus isolation in traditional cell cultures (monolayer cultures) 1 . Primary culture 2 . Semi - continuous cell culture 3 . Continuous cell culture Primary cultures Viable cell suspensions may be obtained by dissociating tissues or organs, e.g. human amnion, with trypsin, collagenase or other enzymes. Semi continuous cell cultures (cell strains ) – Semi continuous cell cultures are established with the successful subculture of primary cell monolayers. These cultures consist mostly of spindle shaped fibroblast cells. Established from human embryonic tissue, or neonatal foreskin. .
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