193x Filetype PPTX File size 1.92 MB Source: fac.ksu.edu.sa
Chlorophyll Fluorescence Plant chlorophyll fluorescence technology was developed in the 1990s. The variation in fluorescence can be shown by false color images or as numerical values from the photochemical reaction kinetics (quantum yield, fluorescence decline, non-photosynthetic quenching). Chlorophyll Fluorescence Photosynthesis belongs to ones of the oldest photo-physical and biochemical processes on Earth. A large progress in the research of photosynthesizing organisms was achieved at the end of 20th century by the introduction of modern optical methods and techniques allowing to study photosynthetic processes ranging from the subcellular up to the plant canopy level. Chlorophyll (Chl) fluorescence: These techniques appeared to be a very powerful tool for studying of photochemical and non-photochemical processes within thylakoid membranes, chloroplasts, plant tissues, or whole plants. Chlorophyll Fluorescence Photosynthesis is the primary process by which plants synthesize food and maintain energy. As part of that process some energy is released as light or fluorescence, commonly in the chlorophyll pigment areas in the leaf. This chlorophyll fluorescence can be measured using the Chlorophyll Fluorescence meter to provide an inside look at the efficiency of the photosynthetic process or its disruption due to stress related factors. Applications: • Photosynthesis research • Plant ecology • Stress physiology • Terrestrial / aquatic plants • Information from whole leaf to single cells • Detecting phytotoxic compounds Chlorophyll fluorescence measurements in plants by : J. Leipner Light energy that is absorbed by chlorophyll in a leaf can undergo three fates: a) It can be used to drive photosynthesis (photochemistry). b) It can be dissipated as heat. c) It can be re-emitted as red fluorescence. Absorption and emission spectrum of chlorophyll Chlorophyll fluorescence measurements in plants by : J. Leipner These three process occur in competition. Any increase in the efficiency of one process will result in a decrease in the yield of the other two. Therefore, determining the yield of chlorophyll fluorescence will give information about changes in the efficiency of photochemistry and heat dissipation. Possible fates of excited chlorophyll
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