Need to Add to part 2 or 3
  A. Chip-seq
  B. Deep sequencing for expression profiling
  C.  Illumina? movie
                    Genomics Summary
  A. Microarrays: expression profiling and other uses
  B. Global Gene Knockouts 
  C.  Global protein localization in yeast 
  D. Global complex identification in yeast 
  E.  Global two-hybrid analysis in yeast and other 
      organisms
  F.  RNAi
  G. Transgenics, gene “knock-outs” (genetics not 
      genomics)
  H. Human Genome Project, Next Generation 
      Sequencing, and Comparative Genomics
     Yeast “Knockout” Library
         Delete YFG
     Delete all genes (individually)
     Disruption of “All” Yeast Genes
  • Approx 6000 genes
  • Make 6000 sets of disruption primers
  • Disrupt each gene in a diploid
  • Dissect all 6000 diploids
    – Identify set of essential genes
    – Identify set of non-essential genes
                     Yeast “Knockout” Library
      •  Delete one copy of each gene in diploid
          – 5,916 “genes” deleted
          – 5,916 diploid strains constructed
      •  Dissect to determine if gene is essential
          – 1,105 genes = essential
          – 18.7% of genes = essential
      •  Construct an ordered library of haploids for non-
         essential genes
          – 4,811 mutant strains in library