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Agilent Technologies is committed to the educational community and is willing to provide access to company-owned material contained herein. This slide set is created by Agilent Technologies. The usage of the slides is limited to teaching purpose only. These materials and the information contained herein are accepted “as is” and Agilent makes no representations or warranties of any kind with respect to the materials and disclaims any responsibility for them as may be used or reproduced by you. Agilent will not be liable for any damages resulting from or in connection with your use, copying or disclosure of the materials contained herein. You agree to indemnify and hold Agilent harmless for any claims incurred by Agilent as a result of your use or reproduction of these materials. In case pictures, sketches or drawings should be used for any other purpose please contact Agilent Technologies a priori. For teaching purpose only 8/28/22 © Agilent Technologies, Inc. 2016 2 Introduction Spectroscopy is the study of the interaction between matter and electro- magnetic radiation. Historically, spectroscopy originated through the study of visible light dispersed according to its wavelength, by a prism. Later the concept was expanded greatly to comprise any interaction with radiative energy as a function of its wavelength or frequency. Spectroscopic data is often represented by a spectrum, a plot of the response of interest as a function of wavelength or frequency. • Spectrum (Latin): ghost • Skopos (Greek): watcher • Spectroscopist = ghost watcher For teaching purpose only 8/28/22 © Agilent Technologies, Inc. 2016 3 Table of Contents Historical Background Key Parameters • Early History of Optical Spectra • Wavelength and Frequency • 1666 Observation of Visible Spectrum • Absorption and Emission • 1802 Fraunhofer Absorption Lines • Light Absorbed vs. Energy Levels • Kirchhoff & Bunsen’s Emission • Characteristics of Atomic Spectra Experiment • Absorbance and Transmittance • Kirchhoff & Bunsen’s Absorption • Absorbance: Concentration Experiment Relationship • Beer-Bouguer-Lambert Law Definitions • Spectroscopy and Spectrometer • Electromagnetic Spectrum • Light For teaching purpose only 8/28/22 © Agilent Technologies, Inc. 2016 4 Historical Background Early History of Optical Spectra 1666 1802 1812 1853 1859 1868 1882 Sir Isaac William Hyde Joseph von August Beer Gustav Anders J. Abney & Newton Wollaston Fraunhofer recognizes Kirchhoff & Angstrom Festing discovers identifies dark studies these the Robert measures the obtained solar lines in solar dark lines relationship Bunsen wavelengths infrared spectrum spectrum using a between the observe of about absorption spectroscope absorption of different 1,000 spectra for light and colors from Fraunhofer over 50 concentration elements lines compounds heated to incandescenc e ToC For teaching purpose only 8/28/22 © Agilent Technologies, Inc. 2016 5 Historical Background 1666 Observation of Visible Spectrum Sir Isaac Newton`s Experiment Sir Isaac Newton,1642-1726 English physicist and mathematician Source: Wikipedia ToC For teaching purpose only 8/28/22 © Agilent Technologies, Inc. 2016 6
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