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picture1_Genome Sequencing Slideshare 66934 | Wgs For Epidemiologists


 169x       Filetype PPTX       File size 1.82 MB       Source: nyfoodsafety.cals.cornell.edu


File: Genome Sequencing Slideshare 66934 | Wgs For Epidemiologists
objectives microbial genomes common isolate identification techniques using molecular biology whole genome sequencing wgs example of wgs for outbreak investigation questions microbial genomes genome size varies from 4 56 to ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 3 years ago
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    Objectives
     Microbial genomes
     Common isolate identification techniques 
      using molecular biology
     Whole genome sequencing (WGS)
     Example of WGS for outbreak investigation
     Questions
    Microbial Genomes
     Genome size varies from 4.56 to 5.70 Mb
     How big is 5 Mb???
    Harry Potter Story
     Long story, some big books!
     1,084,440 words in all seven books
     Average word length ~5 letters
     ~5,422,200 letters total in box set
     E.coli genomes range from 4.56 to 5.70 Mb
     A single E. coli genome @ 1 box set
     Single human genome @ 1,000 box sets!!!
  PFGE (Pulsed Field Gel 
  Electrophoresis)
    What do these bands really mean???
     PFGE (Pulsed Field Gel 
     Electrophoresis)
                1 site     2 sites    3 sites     2 sites     4 sites
                5 Mb       5 Mb        5 Mb       5 Mb        5 Mb
     b
     M  5Mb
      
     n
     i
      
     e
     z
     i
     s
      
     e
     m  1Mb
     o
     n
     e
     G 0.5Mb
                                                      Restriction enzyme site
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...Objectives microbial genomes common isolate identification techniques using molecular biology whole genome sequencing wgs example of for outbreak investigation questions size varies from to mb how big is harry potter story long some books words in all seven average word length letters total box set e coli range a single human sets pfge pulsed field gel electrophoresis what do these bands really mean site sites b m n i z s o g restriction enzyme...

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