169x Filetype PPTX File size 1.82 MB Source: nyfoodsafety.cals.cornell.edu
Objectives Microbial genomes Common isolate identification techniques using molecular biology Whole genome sequencing (WGS) Example of WGS for outbreak investigation Questions Microbial Genomes Genome size varies from 4.56 to 5.70 Mb How big is 5 Mb??? Harry Potter Story Long story, some big books! 1,084,440 words in all seven books Average word length ~5 letters ~5,422,200 letters total in box set E.coli genomes range from 4.56 to 5.70 Mb A single E. coli genome @ 1 box set Single human genome @ 1,000 box sets!!! PFGE (Pulsed Field Gel Electrophoresis) What do these bands really mean??? PFGE (Pulsed Field Gel Electrophoresis) 1 site 2 sites 3 sites 2 sites 4 sites 5 Mb 5 Mb 5 Mb 5 Mb 5 Mb b M 5Mb n i e z i s e m 1Mb o n e G 0.5Mb Restriction enzyme site
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