A THESIS
                FOR THE DEGREE OF MASTER OF SCIENCE
        Effects of Ecklonia cava on Plasma and Liver Lipids,
        Platelet Aggregation and RBC Membrane Stability in
                     Rats Fed Cholesterol Diet
                           Wan-Ting Yeh
                DEPARTMENT OF FOOD SCIENCE AND NUTRITION
                         GRADUATE SCHOOL
                      JEJU NATIONAL UNIVERSITY
                              2010. 2.
        Effects of Ecklonia cava on Plasma and Liver Lipids,
         Platelet Aggregation and RBC Membrane Stability in
                      Rats Fed Cholesterol Diet
                           指導敎授 姜 晶 淑
                              葉 宛 婷
                 이論文을理學碩士學位論文으로提出함
                              2010 年 2 月
                葉宛婷의 理學 碩士學位 論文을 認准함
                                                         印
                            審査委員長_______________________
                                                         印
                            委      員______________________
                                                         印
                            委      員______________________
                           濟州大學校大學院
                              2010 年 2 月
                                 CONTENTS
         ABSTRACT·········································································································ⅲ
         LIST OF TABLES······························································································ⅴ
         LIST OF FIGURES·····························································································ⅵ
         Ⅰ. INTRODUCTION·····························································································1
         Ⅱ. LITERATURE REVIEW··················································································3
           1. Antioxidant and physiological functions···························································3
           2. Plant fiber and physiological roles··································································4
         Ⅲ. MATERIAL & METHOD
           1. Animal and diet······························································································7
           2. Analysis of sample·························································································9
             1) Whole blood platelet aggregation······························································9
             2) Plasma HDL-cholesterol, Total cholesterol and Triglyceride·······················9
             3) Liver Total cholesterol & Triglyceride······················································10
             4) Erythrocyte Na-leak···············································································12
             5) PRP TBARS production···········································································14
             6) Liver TBARS production··········································································14
             7) Protein analysis······················································································14
             8) Measurement of hemolysis······································································15
             9) Statistical analysis··················································································15
                                   - i -
          Ⅳ. RESULTS ＆ DISCUSSIONS
           1. Weight gain and food efficiency·································································16
           2. Plasma total-cholesterol, HDL-cholesterol, triglyceride and glucose········19
           3. Liver cholesterol and triglyceride···························································22
           4. Whole blood platelet aggregation····························································24
           5. Hemolysis and erythrocyte Na leak························································27
           6. Liver and PRP TBARS production··························································30
           7. GOT & GPT···························································································32
          Ⅴ. CONCLUSION···························································································34
          Ⅵ. REFERENCES···························································································36
                                      - ii -